What are the outcomes when embryos frozen without biopsy are thawed, biopsied for PGT-A, and re frozen?

We see approximately 15% of embryos that are thawed, biopsied, and refrozen undergo degeneration during the process. 85% of the embryos survive the process. At a mean age at oocyte retrieval of approximately 35 years old we see a 70% euploid rate.

When embryos that are initially frozen without biopsy are thawed, biopsied, re frozen, found to be euploid, and ultimately transferred, we see a 30-40% live birth rate (compared to 55% for embryos biopsied and frozen from the start).

Overall there is about a 30-40% loss of reproductive potential in the thaw biopsy and re cryo process. About half of that manifests as embryo degradation (during the thaw biopsy cryo or the subsequent pre-transfer thaw) and the other half as lower pregnancy rates from embryos ultimately transferred.